FLOW CYTOMETRY SERVICE

What is flow cytometry?

Flow cytometry is defined as the simultaneous measurement of phenotypic, biochemical or molecular characteristics of cells as they flow past a laser beam.

The created light signals are collected by detectors and translated into electronic signals which are recorded and analysed by a computer programme.

It is possible to obtain different kinds of information like cell size, internal complexity, internal and external cell components and DNA content by analysis of light scatter from cells and fluorescence from the cells themselves or molecules attached to them.

Research using flow cytometer

The Beckman Coulter Gallios flow cytometer of the unit has two solid-state lasers in standard red (638 nm) and blue (488 nm). It is suitable for a wide range of research applications like phenotypic analysis of intracellular or extracellular markers, DNA content determination, purification of cell populations based on internal or external markers etc.

FLOW CYTOMETRY SERVICE AT THE BAT UNIT

Location and contact

Instituto de Investigación en Ciencias de la Alimentación (CIAL)
c/ Nicolás Cabrera, 9
28049 Madrid

Bioanalytical and Preparative Techniques Laboratory (L4G)
Telephone: 91 0017900 (ext 805)
Responsible technician: Juan Antonio Rodriguez (jantonio.rodriguez.gutierrez@csic.es)

Functions of the service

This service is offered in collaboration with the Centro de Biología Molecular Severo Ochoa (CBMSO) of the UAM Campus. Users from both institutes book online. The BAT Unit ensures proper maintenance and periodical calibration of the instrument as well as related management (user authorization, invoicing etc.)

Normas de funcionamiento y uso del servicio

BOOK (in Spanish)

BOOKING GUIDE (in Spanish)